Sandwich ELISA standard curve of MP00752-3, Human VE-cadherin/CD144 Recombinant Matched Antibody Pair - PBS only. 83766-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1200. 83766-4-PBS was HRP conjugated as the detection antibody. Range: 0.78-50 ng/mL

Serum of sixteen humans was measured. The human VE-cadherin/CD144 concentration of detected samples was determined to be 3,148.2 ng/mL with a range of 2,070.2 - 4,839.1 pg/mL.

HUVEC were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human VE-cadherin/CD144, and measured 175.74 ng/mL. JAR were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human VE-cadherin/CD144, and measured 4.13 ng/mL.

Cytometric bead array standard curve of MP00752-3, VE-cadherin/CD144 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83766-1-PBS. Detection antibody: 83766-4-PBS. Standard: Eg1200. Range: 0.78-100 ng/mL.