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验证数据展示

  • WB analysis using 83769-2-RR (same clone as 83769-2-PBS)

    WB analysis using 83769-2-RR (same clone as 83769-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83769-2-RR (ENOSF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83769-2-PBS in a different storage buffer formulation.

  • FC experiment of Hela using 83769-2-RR (same clone as 83769-2-PBS)

    FC experiment of Hela using 83769-2-RR (same clone as 83769-2-PBS)

    1x10^6 Hela cells were intracellularly stained with 0.25 ug ENOSF1 Recombinant antibody (83769-2-RR, Clone:240796D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83769-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00758-1

    Cytometric bead array standard curve of MP00758-1

    Cytometric bead array standard curve of MP00758-1, ENOSF1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83769-1-PBS. Detection antibody: 83769-2-PBS. Standard: Ag34665. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83769-2-RR

    Affinity and Kinetic Characterization of 83769-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83769-2-RR against Human ENOSF1 were performed. The affinity constant is below 1 pM.

ENOSF1 Recombinant antibody, PBS Only (Detector)

ENOSF1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83769-2-PBS

产品说明书

CloneNo. 240796D2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

TYMSAS, rTS, L-fuconate dehydratase, enolase superfamily member 1, EC:4.2.1.68

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83769-2-PBS targets ENOSF1 as part of a matched antibody pair:

MP00758-1: 83769-1-PBS capture and 83769-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 ENOSF1 fusion protein Ag34665 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 enolase superfamily member 1
别名 TYMSAS, rTS, L-fuconate dehydratase, enolase superfamily member 1, EC:4.2.1.68
计算分子量 50 kDa
观测分子量 49 kDa
GenBank蛋白编号BC001285
基因名称 ENOSF1
Gene ID (NCBI) 55556
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7L5Y1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ENOSF1 (Mitochondrial enolase superfamily member 1) is also named as RTS and TYMSAS. The ENOSF1 gene, responsible for encoding the protein mitochondrial enolase superfamily member 1 (ENOF1), exhibits three distinct isoforms. One of these isoforms has been identified as an l-fuconate dehydratase, actively participating in the catabolism of L-fucose, a sugar integral to the carbohydrate composition of cellular glycoproteins (PMID: 38203276). The ENOSF1 transcript has been shown to act as antisense RNA and inhibit TYMS expression (PMID: 35931051).