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验证数据展示

  • WB analysis using 83771-5-RR

    WB analysis using 83771-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83771-5-RR (SMUG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of U-2 OS using 83771-5-RR

    FC experiment of U-2 OS using 83771-5-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug SMUG1 Recombinant antibody (83771-5-RR, Clone:240817E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83771-5-RR

    Affinity and Kinetic Characterization of 83771-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83771-5-RR against Human SMUG1 were performed. The affinity constant is below 1 pM.

SMUG1 Recombinant antibody

SMUG1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83771-5-RR
CloneNo. 240817E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

UNG3, Single-strand selective monofunctional uracil DNA glycosylase, HMUDG, FDG, EC:3.2.2.-

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMOLT-4 cells, K-562 cells, HEK-293 cells, COLO 320 cells, HeLa cells
Positive FC (Intra) detected inU-2 OS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83771-5-RR targets SMUG1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen SMUG1 fusion protein Ag13934 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name single-strand-selective monofunctional uracil-DNA glycosylase 1
Synonyms UNG3, Single-strand selective monofunctional uracil DNA glycosylase, HMUDG, FDG, EC:3.2.2.-
Calculated Molecular Weight 177 aa, 20 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC000417
Gene Symbol SMUG1
Gene ID (NCBI) 23583
RRIDAB_3671365
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ53HV7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UNG(Uracil-DNA glycosylase) removes uracil in DNA resulting from deamination of cytosine or replicative incorporation of dUMP instead of dTMP. Thus, UNG plays a role in suppressing GC-to-AT transition mutations.The UNG gene encodes 2 isoforms that are individually targeted to the mitochondria and the nucleus(PMID:12369930).Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5).

实验方案

Product Specific Protocols
WB protocol for SMUG1 antibody 83771-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols