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HNRNPA2B1 Recombinant antibody

HNRNPA2B1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240831E3

Cat no : 83773-7-RR

Print datasheet

Synonyms

HNRPA2, HNRNPB1, HNRNPA2, hnRNP A2/B1, FLJ22720

验证数据展示

  • WB analysis using 83773-7-RR

    WB analysis using 83773-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83773-7-RR (HNRNPA2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83773-7-RR

    IF Staining of HeLa using 83773-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA2B1 antibody (83773-7-RR, Clone: 240831E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of mouse colon using 83773-7-RR

    IHC staining of mouse colon using 83773-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83773-7-RR (HNRNPA2B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HNRNPA2B1 antibody included
  • FC experiment of HeLa using 83773-7-RR

    FC experiment of HeLa using 83773-7-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HNRNPA2B1 Recombinant antibody (83773-7-RR, Clone:240831E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

  • Affinity and Kinetic Characterization of 83773-7-RR

    Affinity and Kinetic Characterization of 83773-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83773-7-RR against Human HNRNPA2B1 were performed. The affinity constant is 5.04 nM.

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, K-562 cells, HL-60 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HNRNPA2B1 Ready-To-Use IHC Kit. HNRNPA2B1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83773-7-RR is HNRNPA2B1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HNRNPA2B1 fusion protein Ag6563 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heterogeneous nuclear ribonucleoprotein A2/B1
Synonyms HNRPA2, HNRNPB1, HNRNPA2, hnRNP A2/B1, FLJ22720
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 36 kDa, 38 kDa
GenBank Accession NumberBC000506
Gene Symbol HNRNPA2B1
Gene ID (NCBI) 3181
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP22626
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HnRNPA2B1 is one of the heterogenous nuclear ribonucleoproteins (HnRNPs), which regulate the splicing and transportation of mRNA. It has a role in packaging nascent mRNA, alternative splicing, cytoplasmic RNA trafficking, translation, and stabilization. Besides it may involve in telomere maintenance, cell proliferation and differentiation, and glucose transport. Gene HURNPA2B1 encodes two proteins, HNRNPA2 (36kd) and HNRNPB1 (38kd), by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for HNRNPA2B1 antibody 83773-7-RRDownload protocol
IHC protocol for HNRNPA2B1 antibody 83773-7-RRDownload protocol
IF protocol for HNRNPA2B1 antibody 83773-7-RRDownload protocol
FC protocol for HNRNPA2B1 antibody 83773-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols