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验证数据展示

  • WB analysis using 83784-4-RR

    WB analysis using 83784-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83784-4-RR (Islet 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 83784-4-RR

    IHC staining of mouse brain using 83784-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83784-4-RR (Islet 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 83784-4-RR

    IHC staining of mouse brain using 83784-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83784-4-RR (Islet 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 83784-4-RR

    IF Staining of SH-SY5Y using 83784-4-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Islet 1 antibody (83784-4-RR, Clone: 240833D7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83784-4-RR

    Affinity and Kinetic Characterization of 83784-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83784-4-RR against Human Islet 1 were performed. The affinity constant is below 1 pM.

Islet 1 Recombinant antibody

Islet 1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83784-4-RR

产品说明书

CloneNo. 240833D7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

ISL1, ISLET1, ISL LIM homeobox 1, Isl 1, Insulin gene enhancer protein ISL-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, Y79 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83784-4-RR targets Islet 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Islet 1 fusion protein Ag33541 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ISL LIM homeobox 1
别名 ISL1, ISLET1, ISL LIM homeobox 1, Isl 1, Insulin gene enhancer protein ISL-1
计算分子量 39 kDa
观测分子量 39-45 kDa
GenBank蛋白编号BC031213
基因名称 Islet 1
Gene ID (NCBI) 3670
RRIDAB_3671375
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61371
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts. Ubiquitinated probably by WWP1 E3 ubiquitin ligase; ubiquitination is followed by protein degradation.

实验方案

Product Specific Protocols
WB protocol for Islet 1 antibody 83784-4-RRDownload protocol
IHC protocol for Islet 1 antibody 83784-4-RRDownload protocol
IF protocol for Islet 1 antibody 83784-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols