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Islet 1 Recombinant antibody

Islet 1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240833D7

Cat no : 83784-4-RR

Print datasheet

Synonyms

ISL1, ISLET1, ISL LIM homeobox 1, Isl 1, Insulin gene enhancer protein ISL-1

验证数据展示

  • WB analysis using 83784-4-RR

    WB analysis using 83784-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83784-4-RR (Islet 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 83784-4-RR

    IF Staining of SH-SY5Y using 83784-4-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Islet 1 antibody (83784-4-RR, Clone: 240833D7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inSH-SY5Y cells, Y79 cells
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83784-4-RR is Islet 1 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Islet 1 fusion protein Ag33541 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ISL LIM homeobox 1
Synonyms ISL1, ISLET1, ISL LIM homeobox 1, Isl 1, Insulin gene enhancer protein ISL-1
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberBC031213
Gene Symbol Islet 1
Gene ID (NCBI) 3670
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61371
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts. Ubiquitinated probably by WWP1 E3 ubiquitin ligase; ubiquitination is followed by protein degradation.

实验方案

Product Specific Protocols
WB protocol for Islet 1 antibody 83784-4-RRDownload protocol
IF protocol for Islet 1 antibody 83784-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols