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SON Recombinant antibody

SON Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240845B7

Cat no : 83787-5-RR

Print datasheet

Synonyms

SON DNA binding protein, KIAA1019, HSPC312, DBP5, C21orf50

验证数据展示

  • WB analysis using 83787-5-RR

    WB analysis using 83787-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83787-5-RR (SON antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 83787-5-RR

    IF Staining of U2OS using 83787-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SON antibody (83787-5-RR, Clone: 240845B7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of U2OS using 83787-5-RR

    IF Staining of U2OS using 83787-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SON antibody (83787-5-RR, Clone: 240845B7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83787-5-RR

    FC experiment of HeLa using 83787-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SON Recombinant antibody (83787-5-RR, Clone:240845B7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83787-5-RR

    Affinity and Kinetic Characterization of 83787-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83787-5-RR against Human SON were performed. The affinity constant is 0.591 nM.

经过测试的应用

Positive WB detected inHeLa cells, L02 cells, human testis tissue
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy SON Ready-To-Use IHC Kit. SON primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83787-5-RR is SON Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen SON fusion protein Ag27620 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SON DNA binding protein
Synonyms SON DNA binding protein, KIAA1019, HSPC312, DBP5, C21orf50
Calculated Molecular Weight 264 aa
Observed Molecular Weight 250-300 kDa
GenBank Accession NumberNM_001291411
Gene Symbol SON
Gene ID (NCBI) 6651
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP18583
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SON, also called C21orf50 and DBP5, is a nuclear speckle-localized protein that shares homology with pre-mRNA splicing accessory factors (PMID: 10950926). It is an RNA-binding protein that acts as an mRNA splicing cofactor by promoting efficient splicing of transcripts that possess weak splice sites, and specifically promotes splicing of many cell-cycle and DNA-repair transcripts that possess weak splice sites, such as TUBG1, KATNB1, TUBGCP2, AURKB, PCNT, AKT1, RAD23A, and FANCG (PMID: 20581448; 21504830).

实验方案

Product Specific Protocols
WB protocol for SON antibody 83787-5-RRDownload protocol
IF protocol for SON antibody 83787-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols