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验证数据展示

  • WB analysis of A549 using 83790-1-RR

    WB analysis of A549 using 83790-1-RR

    A549 cells were subjected to SDS PAGE followed by western blot with 83790-1-RR (EPAS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat kidney using 83790-1-RR

    WB analysis of rat kidney using 83790-1-RR

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 83790-1-RR (EPAS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83790-1-RR

    Affinity and Kinetic Characterization of 83790-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83790-1-RR against Human HIF2α/EPAS1 were performed. The affinity constant is below 1 pM.

HIF2α/EPAS1 Recombinant antibody

HIF2α/EPAS1 Recombinant Antibody for WB, ELISA
Cat No. 83790-1-RR

产品说明书

CloneNo. 240736F2

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, ELISA

别名

EPAS1, Endothelial PAS domain-containing protein 1, ECYT4, Class E basic helix-loop-helix protein 73, bHLHe73

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, rat kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83790-1-RR targets HIF2α/EPAS1 in WB, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, rat
免疫原 HIF2α/EPAS1 fusion protein Ag15199 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 endothelial PAS domain protein 1
别名 EPAS1, Endothelial PAS domain-containing protein 1, ECYT4, Class E basic helix-loop-helix protein 73, bHLHe73
计算分子量 96 kDa
观测分子量 100-120 kDa
GenBank蛋白编号BC051338
基因名称 HIF2α
Gene ID (NCBI) 2034
RRIDAB_3671381
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99814
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIF2A, also named as EPAS1, is a 870 amino acid protein, which is expressed in most tissues, with highest levels in placenta, lung and heart. HIF2A colocalizes with HIF3A in the nucleus and speckles. HIF2A as a transcription factor involves in the induction of oxygen regulated genes. HIF2A binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. HIF2A regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. HIF2A may also play a role in the formation of the endothelium that gives rise to the blood brain barrier. The calculated molecular weight of HIF2A is 96 kDa, but In normoxia, HIF2A is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization. The modified HIf2A is about 100-120 kDa.

实验方案

Product Specific Protocols
WB protocol for HIF2α/EPAS1 antibody 83790-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols