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Species Reactivity
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Conjugate
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验证数据展示

  • FC experiment of Jurkat using 83808-2-RR

    FC experiment of Jurkat using 83808-2-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-2-RR, Clone:240684B8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of MCF-7 using 83808-2-RR

    IF Staining of MCF-7 using 83808-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (83808-2-RR, Clone: 240684B8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

GATA3 Recombinant antibody

GATA3 Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83808-2-RR

产品说明书

CloneNo. 240684B8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 240684B8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inJurkat cells, U-937 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83808-2-RR targets GATA3 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 GATA3 fusion protein Ag0174 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GATA binding protein 3
别名 Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 240684B8
计算分子量 443 aa, 48 kDa
GenBank蛋白编号BC003070
基因名称 GATA3
Gene ID (NCBI) 2625
RRIDAB_3671396
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP23771
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression. This is a rabbit polyclonal antibody raised against part of C-terminus of human GATA3.

实验方案

Product Specific Protocols
IF protocol for GATA3 antibody 83808-2-RRDownload protocol
FC protocol for GATA3 antibody 83808-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols