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验证数据展示

  • WB analysis using 83809-1-RR

    WB analysis using 83809-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83809-1-RR (TP53BP1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83809-1-RR

    IHC staining of human ovary cancer using 83809-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83809-1-RR (TP53BP1-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 83809-1-RR

    IF Staining of HeLa using 83809-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TP53BP1-Specific antibody (83809-1-RR, Clone: 240638C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83809-1-RR

    FC experiment of HeLa using 83809-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TP53BP1-Specific Recombinant antibody (83809-1-RR, Clone:240638C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

TP53BP1-Specific Recombinant antibody

TP53BP1-Specific Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83809-1-RR

产品说明书

CloneNo. 240638C6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

TP53BP1, p53-binding protein 1, p53 binding protein 1, p202, FLJ41424

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83809-1-RR targets TP53BP1-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor protein p53 binding protein 1
别名 TP53BP1, p53-binding protein 1, p53 binding protein 1, p202, FLJ41424
计算分子量 214 kDa
观测分子量450 kDa
GenBank蛋白编号NM_005657
基因名称 TP53BP1
Gene ID (NCBI) 7158
RRIDAB_3671397
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ12888
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TP53BP1, also named as p53BP1, p202, 53BP1 and FLJ41424, may have a role in checkpoint signaling during mitosis. TP53BP1 enhances TP53-mediated transcriptional activation. TP53BP1 plays a role in the response to DNA damage.

实验方案

Product Specific Protocols
WB protocol for TP53BP1-Specific antibody 83809-1-RRDownload protocol
IHC protocol for TP53BP1-Specific antibody 83809-1-RRDownload protocol
IF protocol for TP53BP1-Specific antibody 83809-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols