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验证数据展示

  • IF Staining of LNCaP using 83824-5-RR

    IF Staining of LNCaP using 83824-5-RR

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using CNNM4 antibody (83824-5-RR, Clone: 240873C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of MCF-7 using 83824-5-RR

    IF Staining of MCF-7 using 83824-5-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CNNM4 antibody (83824-5-RR, Clone: 240873C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83824-5-RR

    Affinity and Kinetic Characterization of 83824-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83824-5-RR against Human CNNM4 were performed. The affinity constant is below 1 pM.

CNNM4 Recombinant antibody

CNNM4 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 83824-5-RR

产品说明书

CloneNo. 240873C1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

KIAA1592, Cyclin-M4, cyclin M4, Ancient conserved domain-containing protein 4, ACDP4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inLNCaP cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83824-5-RR targets CNNM4 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CNNM4 fusion protein Ag5121 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin M4
别名 KIAA1592, Cyclin-M4, cyclin M4, Ancient conserved domain-containing protein 4, ACDP4
计算分子量 87 kDa
GenBank蛋白编号BC063295
基因名称 CNNM4
Gene ID (NCBI) 26504
RRIDAB_3671409
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ6P4Q7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ancient conserved domain-containing protein 4/cyclin M4 (CNNM4), a membrane protein that stimulates Mg2+ efflux, binds phosphatase of regenerating liver (PRL), which is frequently overexpressed in malignant human cancers (PMID: 25347473). CNNM4 is predominant in the brain, bone marrow, immune system, and especially abundant in the intestinal tract. CNNM4 localizes at the basolateral membrane of the intestinal epithelia and mediates transcellular Mg2+ transport (PMID: 27006114).

实验方案

Product Specific Protocols
IF protocol for CNNM4 antibody 83824-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols