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PTPRS Recombinant antibody

PTPRS Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241005B6

Cat no : 83833-5-RR

Print datasheet

Synonyms

Receptor-type tyrosine-protein phosphatase S, R PTP sigma, R PTP S, PTPSIGMA, EC:3.1.3.48

验证数据展示

  • WB analysis using 83833-5-RR

    WB analysis using 83833-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83833-5-RR (rPTPσ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83833-5-RR

    Affinity and Kinetic Characterization of 83833-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83833-5-RR against Human PTPRS were performed. The affinity constant is 68.9 pM.

经过测试的应用

Positive WB detected inA431 cells, SH-5Y5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83833-5-RR is PTPRS Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, S
Synonyms Receptor-type tyrosine-protein phosphatase S, R PTP sigma, R PTP S, PTPSIGMA, EC:3.1.3.48
Calculated Molecular Weight217 kDa
Observed Molecular Weight140 kDa, 217 kDa
GenBank Accession NumberNM_002850
Gene Symbol PTPRS
Gene ID (NCBI) 5802
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13332
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tyrosine phosphatase receptor type S (PTPRS, also known as R-PTP-S, PTPSIGMA, and R-PTP-sigma) is an integral membrane protein. It can promote oligomerization by binding to large heparan sulfate proteoglycan structures, and interact with NTRK1/3, PPFIA1/2/3 (PMID: 25385546; 8524829; 9624153). The non-glycosylated molecular weight of PTPRS is 214 kDa, and the extracellular domain can be cleaved near the cell membrane by an endoprotease to generate a glycated 140 kDa protein. Variations and mutations of PTPRS are associated with Alzheimer's disease and cholangiocarcinoma (PMID: 38766183; 35991009).

实验方案

Product Specific Protocols
WB protocol for PTPRS antibody 83833-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols