验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HeLa cells, HEK-293T cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83856-5-RR targets MIOS in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | MIOS fusion protein Ag15148 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | missing oocyte, meiosis regulator, homolog (Drosophila) |
| 别名 | Missing oocyte meiosis regulator homolog, GATOR2 complex protein MIOS, 240832B9 |
| 计算分子量 | 875 aa, 99 kDa |
| 观测分子量 | 82 kDa |
| GenBank蛋白编号 | BC005883 |
| 基因名称 | MIOS |
| Gene ID (NCBI) | 54468 |
| RRID | AB_3671441 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9NXC5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mechanistic target of rapamycin complex 1 (mTORC1) controls growth by regulating anabolic and catabolic processes in response to environmental cues, including nutrients. Amino acids signal to mTORC1 through the Rag GTPases, which are regulated by several protein complexes, including GATOR1 and GATOR2. GATOR2, which has five components (WDR24, MIOS, WDR59, SEH1L, SEC13), is required for amino acids to activate mTORC1. PMID: 35831510
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MIOS antibody 83856-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |