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验证数据展示

  • WB analysis using 83858-3-RR (same clone as 83858-3-PBS)

    WB analysis using 83858-3-RR (same clone as 83858-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83858-3-RR (PYCARD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83858-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 83858-3-RR (same clone as 83858-3-PBS)

    IHC staining of mouse colon using 83858-3-RR (same clone as 83858-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83858-3-RR (ASC/TMS1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83858-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 83858-3-RR (same clone as 83858-3-PBS)

    IHC staining of mouse colon using 83858-3-RR (same clone as 83858-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83858-3-RR (ASC/TMS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83858-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00782-1

    Cytometric bead array standard curve of MP00782-1

    Cytometric bead array standard curve of MP00782-1, MOUSE ASC/TMS1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83858-3-PBS. Detection antibody: 83858-1-PBS. Standard: Ag32519. Range: 0.47-60 ng/mL.

  • Cytometric bead array standard curve of MP00782-2

    Cytometric bead array standard curve of MP00782-2

    Cytometric bead array standard curve of MP00782-2, MOUSE ASC/TMS1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83858-3-PBS. Detection antibody: 83858-4-PBS. Standard: Ag32519. Range: 0.47-60 ng/mL.

  • Affinity and Kinetic Characterization of 83858-3-RR

    Affinity and Kinetic Characterization of 83858-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83858-3-RR against Mouse PYCARD were performed. The affinity constant is 3.58 nM.

Mouse ASC/TMS1 Recombinant antibody, PBS Only (Capture)

ASC/TMS1 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 83858-3-PBS

产品说明书

CloneNo. 240908D5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

Pycard, PYD and CARD domain containing, masc, CARD5, Asc

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83858-3-PBS targets ASC/TMS1 as part of a matched antibody pair:

MP00782-1: 83858-3-PBS capture and 83858-1-PBS detection (validated in Cytometric bead array)

MP00782-2: 83858-3-PBS capture and 83858-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 mouse
免疫原 ASC/TMS1 fusion protein Ag32519 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PYD and CARD domain containing
别名 Pycard, PYD and CARD domain containing, masc, CARD5, Asc
计算分子量21KD
观测分子量21-25 kDa
GenBank蛋白编号NM_023258
基因名称 Pycard
Gene ID (NCBI) 66824
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9EPB4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PYCARD (PYD and CARD domain containing), also known as ASC and CARD5. The calculated molecular weight of PYCARD is 21 kDa. It is located in cytosol, extracellular region and nucleus. PYCARD is also expressed in several structures, including alimentary system; brain; heart; hemolymphoid system gland; and urinary system. The gene enables identical protein binding activity and protein dimerization activity. Involved in several processes, including activation of cysteine-type endopeptidase activity; positive regulation of cytokine production; and regulation of defense response. It acts upstream of or within several processes, including defense response to Gram-positive bacterium; positive regulation of macromolecule metabolic process; and regulation of autophagy. It is also a part of inflammasome complex. Finally, it orthologous to human PYCARD (PYD and CARD domain containing).