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验证数据展示

  • WB analysis of human kidney using 83860-1-RR

    WB analysis of human kidney using 83860-1-RR

    human kidney tissue were subjected to SDS PAGE followed by western blot with 83860-1-RR (FXYD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83860-1-RR

    FC experiment of HepG2 using 83860-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FXYD2 Recombinant antibody (83860-1-RR, Clone:240649C2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83860-1-RR

    Affinity and Kinetic Characterization of 83860-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83860-1-RR against Human FXYD2 were performed. The affinity constant is below 1 pM.

FXYD2 Recombinant antibody

FXYD2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83860-1-RR

产品说明书

CloneNo. 240649C2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Sodium/potassium-transporting ATPase subunit gamma, FXYD domain-containing ion transport regulator 2, FXYD 2, ATP1G1, ATP1C

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman kidney tissue
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83860-1-RR targets FXYD2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 FXYD2 fusion protein Ag34030 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 FXYD domain containing ion transport regulator 2
别名 Sodium/potassium-transporting ATPase subunit gamma, FXYD domain-containing ion transport regulator 2, FXYD 2, ATP1G1, ATP1C
计算分子量 7 kDa
观测分子量 7-10 kDa
GenBank蛋白编号BC013289
基因名称 FXYD2
Gene ID (NCBI) 486
RRIDAB_3671444
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP54710
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FXYD2 (FXYD domain-containing ion transport regulator 2), also known as the gamma-subunit of the NaK-ATPase, belongs to the FXYD family which has been proposed to be the regulators of Na, K-ATPase function by lowering affinities of the system for potassium and sodium. The expression of FXYD2 is most abundant in kidney, while it is also detected in several other tissues like placenta, pancreas, and dorsal root ganglia (DRGs). Three splice variants of FXYD2 have been reported in mouse kidney, namely FXYD2 γa, -γb,and -γc. FXYD2 γa has been identified as a pancreatic beta cell-specific biomarker. This antibody can recognize all three isoforms of FXYD2.

实验方案

Product Specific Protocols
WB protocol for FXYD2 antibody 83860-1-RRDownload protocol
FC protocol for FXYD2 antibody 83860-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols