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RPE65 Recombinant antibody

RPE65 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240518F1

Cat No : 83861-1-RR

Print datasheet

Synonyms

Lutein isomerase, LCA2, EC:5.3.3.22, EC:3.1.1.64, All-trans-retinyl-palmitate hydrolase

验证数据展示

  • WB analysis using 83861-1-RR

    WB analysis using 83861-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83861-1-RR (RPE65 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells, Y79 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83861-1-RR is RPE65 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen RPE65 fusion protein Ag12356 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name retinal pigment epithelium-specific protein 65kDa
Synonyms Lutein isomerase, LCA2, EC:5.3.3.22, EC:3.1.1.64, All-trans-retinyl-palmitate hydrolase
Calculated Molecular Weight61 kDa
Observed Molecular Weight 65 kDa, 61 kDa
GenBank Accession NumberBC075036
Gene Symbol RPE65
Gene ID (NCBI) 6121
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ16518
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPE65(Retinal pigment epithelium-specific 65 kDa protein) is also named as retinoid isomerohydrolase with the calculated molecular weight og 61 kDa and belongs to the carotenoid oxygenase family. It is a microsomal protein with isomerase activity in the retinoid visual cycle that playing a role in the isomerisation of all-trans (vitamin A) to 11-cis retinal, the chromophore of the visual pigments. It also might be involved in retinoid uptake of keratinocytes.

实验方案

Product Specific Protocols
WB protocol for RPE65 antibody 83861-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols