验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, PC-3 cells, Y79 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83861-1-RR targets RPE65 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | RPE65 fusion protein Ag12356 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | retinal pigment epithelium-specific protein 65kDa |
| 别名 | Lutein isomerase, LCA2, EC:5.3.3.22, EC:3.1.1.64, All-trans-retinyl-palmitate hydrolase |
| 计算分子量 | 61 kDa |
| 观测分子量 | 65 kDa, 61 kDa |
| GenBank蛋白编号 | BC075036 |
| 基因名称 | RPE65 |
| Gene ID (NCBI) | 6121 |
| RRID | AB_3671445 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q16518 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPE65(Retinal pigment epithelium-specific 65 kDa protein) is also named as retinoid isomerohydrolase with the calculated molecular weight og 61 kDa and belongs to the carotenoid oxygenase family. It is a microsomal protein with isomerase activity in the retinoid visual cycle that playing a role in the isomerisation of all-trans (vitamin A) to 11-cis retinal, the chromophore of the visual pigments. It also might be involved in retinoid uptake of keratinocytes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPE65 antibody 83861-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |