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PADI2 Recombinant antibody

PADI2 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240763C5

Cat No : 83868-1-RR

Print datasheet

Synonyms

PAD-H19, PAD2, PAD H19, KIAA0994, EC:3.5.3.15

验证数据展示

  • WB analysis using 83868-1-RR

    WB analysis using 83868-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83868-1-RR (PADI2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83868-1-RR

    Affinity and Kinetic Characterization of 83868-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83868-1-RR against Human PADI2 were performed. The affinity constant is 1.01 nM.

经过测试的应用

Positive WB detected inMCF-7 cells, HL-60 cells, mouse brain tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83868-1-RR is PADI2 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PADI2 fusion protein Ag17612 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name peptidyl arginine deiminase, type II
Synonyms PAD-H19, PAD2, PAD H19, KIAA0994, EC:3.5.3.15
Calculated Molecular Weight 665 aa, 75 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC009701
Gene Symbol PADI2
Gene ID (NCBI) 11240
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y2J8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PADI2, also named KIAA0994, PDI2, PAD-H19, and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation-related genes via histone citrullination during diestrus (PMID:20668670).

实验方案

Product Specific Protocols
WB protocol for PADI2 antibody 83868-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols