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4EBP1 Recombinant antibody, PBS Only (Capture/Detector)

4EBP1 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241055C6

Cat No : 83890-1-PBS

Print datasheet

Synonyms

EIF4EBP1, eIF4E-binding protein 1, eIF4E binding protein 1, BP 1, 4E-BP1

验证数据展示

  • Sandwich ELISA standard curve of MP00559-3

    Sandwich ELISA standard curve of MP00559-3

    Sandwich ELISA standard curve of MP00559-3, Human 4EBP1 Recombinant Matched Antibody Pair - PBS only. 83890-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag5056. 83890-2-PBS was HRP conjugated as the detection antibody. Range: 39.06-2500 pg/mL.

  • Sample test of MP00559-3

    Sample test of MP00559-3

    The mean 4EBP1 concentration was determined to be 375.9 ng/mL in A549 cell extract based on a 3.0 mg/mL extract load, 1,210.9 ng/mL in HepG2 cell extract based on a 8.5 mg/mL extract load, 396.7 ng/mL in K-562 cell extract based on a 5.2 mg/mL extract load and 2,456.3 ng/mL in HCT 116 cell extract based on a 6.1 mg/mL extract load.

  • Cytometric bead array standard curve of MP00559-3

    Cytometric bead array standard curve of MP00559-3

    Cytometric bead array standard curve of MP00559-3, 4EBP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83890-1-PBS. Detection antibody: 83890-2-PBS. Standard: Ag5056. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00559-4

    Cytometric bead array standard curve of MP00559-4

    Cytometric bead array standard curve of MP00559-4, 4EBP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83890-4-PBS. Detection antibody: 83890-1-PBS. Standard: Ag5056. Range: 0.625-80 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy EIF4EBP1 Ready-To-Use IHC Kit. EIF4EBP1 primary antibody included.

产品信息

83890-1-PBS targets 4EBP1 as part of a matched antibody pair:

MP00559-3: 83890-1-PBS capture and 83890-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00559-4: 83890-4-PBS capture and 83890-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen 4EBP1 fusion protein Ag5056 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name eukaryotic translation initiation factor 4E binding protein 1
Synonyms EIF4EBP1, eIF4E-binding protein 1, eIF4E binding protein 1, BP 1, 4E-BP1
Calculated Molecular Weight 118 aa, 12 kDa
GenBank Accession NumberBC004459
Gene Symbol EIF4EBP1
Gene ID (NCBI) 1978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13541
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C