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CD25/IL2RA Recombinant antibody, PBS Only (Detector)

CD25/IL2RA Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240906A12

Cat no : 83896-1-PBS

Print datasheet

Synonyms

CD25, 240906A12, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha

验证数据展示

  • WB analysis of MJ using 83896-1-RR (same clone as 83896-1-PBS)

    WB analysis of MJ using 83896-1-RR (same clone as 83896-1-PBS)

    MJ cells were subjected to SDS PAGE followed by western blot with 83896-1-RR (IL2RA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83896-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00837-2

    Cytometric bead array standard curve of MP00837-2

    Cytometric bead array standard curve of MP00837-2, IL2RA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83896-2-PBS. Detection antibody: 83896-1-PBS. Standard: Eg0408. Range: 2.5-40 ng/mL.

  • Affinity and Kinetic Characterization of 83896-1-RR

    Affinity and Kinetic Characterization of 83896-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83896-1-RR against Human CD25/IL2RA were performed. The affinity constant is 31.7 nM.

产品信息

83896-1-PBS targets CD25/IL2RA as part of a matched antibody pair:

MP00837-2: 83896-2-PBS capture and 83896-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 2 receptor, alpha
Synonyms CD25, 240906A12, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha
Calculated Molecular Weight 272 aa, 31 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC156627
Gene Symbol IL2RA
Gene ID (NCBI) 3559
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01589
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present in activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+ Foxp3+ Treg cells (PMID: 22585674).