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验证数据展示

  • WB analysis of A431 using 83898-1-RR

    WB analysis of A431 using 83898-1-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 83898-1-RR (PLEC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83898-1-RR

    WB analysis using 83898-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83898-1-RR (PLEC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 83898-1-RR

    IF Staining of A431 using 83898-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLEC antibody (83898-1-RR, Clone: 240891A10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83898-1-RR

    Affinity and Kinetic Characterization of 83898-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83898-1-RR against Human PLEC were performed. The affinity constant is 52.7 pM.

PLEC Recombinant antibody

PLEC Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83898-1-RR

产品说明书

CloneNo. 240891A10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

PLEC1, Plectin, PLTN, Plectin 1, PLEC1b

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHaCaT cells, A431 cells, HEK-293 cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy PLEC Ready-To-Use IHC Kit. PLEC primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83898-1-RR targets PLEC in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 PLEC fusion protein Ag29418 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 plectin 1, intermediate filament binding protein 500kDa
别名 PLEC1, Plectin, PLTN, Plectin 1, PLEC1b
计算分子量 532 kDa
观测分子量 510 kDa
GenBank蛋白编号NM_201380
基因名称 Plectin
Gene ID (NCBI) 5339
RRIDAB_3671479
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15149
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011)

实验方案

Product Specific Protocols
WB protocol for PLEC antibody 83898-1-RRDownload protocol
IF protocol for PLEC antibody 83898-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols