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验证数据展示

  • WB analysis using 83905-4-RR

    WB analysis using 83905-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83905-4-RR (PLIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83905-4-RR

    Affinity and Kinetic Characterization of 83905-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83905-4-RR against Human Perilipin 1 were performed. The affinity constant is 0.617 nM.

Perilipin 1 Recombinant antibody

Perilipin 1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83905-4-RR

产品说明书

CloneNo. 240994H1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

PLIN, PLIN1, PERI, 240994H1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, MDA-MB-231 cells, 3T3-L1 cells
Planning an IHC experiment? We recommend our IHCeasy PLIN1 Ready-To-Use IHC Kit. PLIN1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83905-4-RR targets Perilipin 1 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 Perilipin 1 fusion protein Ag26426 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 perilipin
别名 PLIN, PLIN1, PERI, 240994H1
计算分子量 56 kDa
观测分子量67 kDa
GenBank蛋白编号NM_001145311
基因名称 Perilipin 1
Gene ID (NCBI) 5346
RRIDAB_3671486
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO60240
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLIN1, also known as Perilipin 1, PLIN, PERI, belongs to the perilipin family. PLIN1 is a hormonally-regulated phosphoprotein that encircles the lipid storage droplet in adipocytes and modulates adipocyte lipid metabolism (PMID: 2040638). PLIN1 is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in inhibiting lipolysis. PLIN1 is detected in adipocytes of white adipose tissue, visceral adipose tissue, and mammary gland (PMID: 27832861, 9521880). The deficiency of PLIN1 causes lipodystrophic syndromes, which disables the optimal accumulation of triglycerides in adipocytes that results in abnormal deposition of lipids in tissues such as skeletal muscle and liver. The storage of lipids in the liver leads to insulin resistance and hypertriglyceridemia (PMID: 21345103).

实验方案

Product Specific Protocols
WB protocol for Perilipin 1 antibody 83905-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols