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FBP2 Recombinant antibody

FBP2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240879C9

Cat no : 83910-5-RR

Print datasheet

Synonyms

Muscle FBPase, Fructose-1,6-bisphosphatase isozyme 2, FBPase 2, EC:3.1.3.11, D-fructose-1,6-bisphosphate 1-phosphohydrolase 2

验证数据展示

  • WB analysis of mouse heart using 83910-5-RR

    WB analysis of mouse heart using 83910-5-RR

    Mouse heart tissue were subjected to SDS PAGE followed by western blot with 83910-5-RR (FBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 83910-5-RR

    IHC staining of mouse skeletal muscle using 83910-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 83910-5-RR

    IHC staining of mouse skeletal muscle using 83910-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 83910-5-RR

    IHC staining of human ovary cancer using 83910-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83910-5-RR (FBP2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse skeletal muscle using 83910-5-RR

    IF Staining of mouse skeletal muscle using 83910-5-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using FBP2 antibody (83910-5-RR, Clone: 240879C9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83910-5-RR

    Affinity and Kinetic Characterization of 83910-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83910-5-RR against Human FBP2 were performed. The affinity constant is 4.67 nM.

经过测试的应用

Positive WB detected inmouse heart tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inmouse skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83910-5-RR is FBP2 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FBP2 fusion protein Ag18245 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name fructose-1,6-bisphosphatase 2
Synonyms Muscle FBPase, Fructose-1,6-bisphosphatase isozyme 2, FBPase 2, EC:3.1.3.11, D-fructose-1,6-bisphosphate 1-phosphohydrolase 2
Calculated Molecular Weight 339 aa, 37 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC113632
Gene Symbol FBP2
Gene ID (NCBI) 8789
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO00757
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBP2, also named muscle FBP, belongs to the FBPase class 1 family. FBP2 is a ubiquitously expressed enzyme of glycogen synthesis from non-carbohydrates, e.g., lactate (glyconeogenesis). However, it also plays a variety of non-enzymatic functions. FBP2 is involved in the regulation of hypoxia-inducible factor 1 (Hif1) stability, cell cycle-dependent events, biogenesis of mitochondria, and protection of the organelles against high reactive oxygen species (ROS)- and high Ca2+-induced stress (PMID: 32962293, PMID: 35626746). The calculated molecular weight of FBP2 is 37 kDa.

实验方案

Product Specific Protocols
WB protocol for FBP2 antibody 83910-5-RRDownload protocol
IHC protocol for FBP2 antibody 83910-5-RRDownload protocol
IF protocol for FBP2 antibody 83910-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols