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验证数据展示

  • IHC staining of mouse cerebellum using 83912-5-RR

    IHC staining of mouse cerebellum using 83912-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83912-5-RR (ANK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse heart using 83912-5-RR

    IF Staining of mouse heart using 83912-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (83912-5-RR, Clone: 240982G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse heart using 83912-5-RR

    IF Staining of mouse heart using 83912-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (83912-5-RR, Clone: 240982G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HEK-293 using 83912-5-RR

    FC experiment of HEK-293 using 83912-5-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Ank2 Recombinant Antibody (83912-5-RR, Clone:240982G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83912-5-RR

    Affinity and Kinetic Characterization of 83912-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83912-5-RR against Human ANK2 were performed. The affinity constant is below 1 pM.

ANK2 Recombinant antibody

ANK2 Uni-rAbTM Recombinant Antibody for IHC, IF-P, FC (Intra), ELISA
Cat No. 83912-5-RR

Print datasheet

CloneNo. 240982G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, FC (Intra), ELISA

Synonyms

Ankyrin 2, ANK 2, 240982G5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83912-5-RR targets ANK2 in IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen ANK2 fusion protein Ag34642 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ankyrin 2, neuronal
Synonyms Ankyrin 2, ANK 2, 240982G5
Calculated Molecular Weight434 kDa
Observed Molecular Weight250-260 kDa
GenBank Accession NumberNM_001148
Gene Symbol ANK2
Gene ID (NCBI) 287
RRIDAB_3671493
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ01484
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Ankyrins, belonging to the adapter protein family, consist of Ankyrin-R (ANK1), Ankyrin-B (ANK2), and Ankyrin-G (ANK3). Initially, ANK2 was considered as a key cytoskeletal-associated protein in the brain. Besides, it was discovered as a pivotal adaptor and scaffolding protein in the heart. Remarkably, recent studies have demonstrated that Ankyrins contribute to the modulation of various biological activities in cancers. (PMID: 36539782)

实验方案

Product Specific Protocols
IHC protocol for ANK2 antibody 83912-5-RRDownload protocol
IF protocol for ANK2 antibody 83912-5-RRDownload protocol
FC protocol for ANK2 antibody 83912-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols