验证数据展示
经过测试的应用
| Positive WB detected in | PMA, Ionomycin and Brefeldin A treated EL-4 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83935-1-RR targets IL-13 in WB, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse, rat |
| 免疫原 | Fusion Protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interleukin 13 |
| 别名 | Il13, interleukin 13, 240771B9 |
| 计算分子量 | 14kDa |
| 观测分子量 | 12 kDa |
| GenBank蛋白编号 | NM_053828.1 |
| 基因名称 | Il13 |
| Gene ID (NCBI) | 116553 |
| RRID | AB_3671517 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P42203 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rat IL-13 is a kind of cytokines, which plays important roles in allergic inflammation and immune response to parasite infection. (PubMed:9366558). Rat IL-13 displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF-alpha through a mechanism that partially involves suppression of NF-kappa-B (PubMed:9366558).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-13 antibody 83935-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |