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验证数据展示

  • WB analysis using 83970-1-RR

    WB analysis using 83970-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83970-1-RR (YTHDC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83970-1-RR

    FC experiment of HepG2 using 83970-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug YTHDC2 Recombinant antibody (83970-1-RR, Clone:241123A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of rat testis using 83970-1-RR

    IHC staining of rat testis using 83970-1-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83970-1-RR (YTHDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with YTHDC2 antibody included
  • IHC staining of rat testis using 83970-1-RR

    IHC staining of rat testis using 83970-1-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83970-1-RR (YTHDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with YTHDC2 antibody included
  • Affinity and Kinetic Characterization of 83970-1-RR

    Affinity and Kinetic Characterization of 83970-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83970-1-RR against Human YTHDC2 were performed. The affinity constant is 0.378 nM.

YTHDC2 Recombinant antibody

YTHDC2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83970-1-RR

产品说明书

CloneNo. 241123A9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), ELISA

别名

YTH domain-containing protein 2, hYTHDC2, EC:3.6.4.13, 3'-5' RNA helicase YTHDC2, 241123A9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, A549 cells, MCF-7 cells, mouse testis tissue
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy YTHDC2 Ready-To-Use IHC Kit. YTHDC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83970-1-RR targets YTHDC2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 YTHDC2 fusion protein Ag26690 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 YTH domain containing 2
别名 YTH domain-containing protein 2, hYTHDC2, EC:3.6.4.13, 3'-5' RNA helicase YTHDC2, 241123A9
观测分子量160 kDa
GenBank蛋白编号BC137285
基因名称 YTHDC2
Gene ID (NCBI) 64848
RRIDAB_3671546
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H6S0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.

实验方案

Product Specific Protocols
WB protocol for YTHDC2 antibody 83970-1-RRDownload protocol
IHC protocol for YTHDC2 antibody 83970-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols