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Conjugate
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验证数据展示

  • WB analysis using 83986-2-RR (same clone as 83986-2-PBS)

    WB analysis using 83986-2-RR (same clone as 83986-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83986-2-RR (NMI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83986-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83986-2-RR (same clone as 83986-2-PBS)

    IF Staining of HepG2 using 83986-2-RR (same clone as 83986-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NMI antibody (83986-2-RR, Clone: 241135A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83986-2-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83986-2-RR (same clone as 83986-2-PBS)

    FC experiment of HepG2 using 83986-2-RR (same clone as 83986-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NMI Recombinant antibody (83986-2-RR, Clone:241135A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83986-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00891-1

    Cytometric bead array standard curve of MP00891-1

    Cytometric bead array standard curve of MP00891-1, NMI Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83986-2-PBS. Detection antibody: 83986-4-PBS. Standard: Ag34759. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00891-3

    Cytometric bead array standard curve of MP00891-3

    Cytometric bead array standard curve of MP00891-3, NMI Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83986-2-PBS. Detection antibody: 83986-3-PBS. Standard: Ag34759. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83986-2-RR

    Affinity and Kinetic Characterization of 83986-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83986-2-RR against Human NMI were performed. The affinity constant is 1.98 nM.

NMI Recombinant antibody, PBS Only (Capture)

NMI Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83986-2-PBS

产品说明书

CloneNo. 241135A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

241135A9, N myc (and STAT) interactor, N myc and STAT interactor, N myc interactor, N-myc and STAT interactor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83986-2-PBS targets NMI as part of a matched antibody pair:

MP00891-1: 83986-2-PBS capture and 83986-4-PBS detection (validated in Cytometric bead array)

MP00891-3: 83986-2-PBS capture and 83986-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 NMI fusion protein Ag34759 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 N-myc (and STAT) interactor
别名 241135A9, N myc (and STAT) interactor, N myc and STAT interactor, N myc interactor, N-myc and STAT interactor
计算分子量 35 kDa
观测分子量35 kDa
GenBank蛋白编号BC001268
基因名称 NMI
Gene ID (NCBI) 9111
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13287
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

N-Myc and STAT Interactor protein (NMI) is an interferon inducible protein that interacts with NMYC and CMYC (members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. It is widely involved in the process of tumor growth, progression, and metastasis.