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验证数据展示

  • WB analysis using 83989-4-RR

    WB analysis using 83989-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83989-4-RR (TPRG1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 83989-4-RR

    IHC staining of mouse brain using 83989-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83989-4-RR (TPRG1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 83989-4-RR

    IHC staining of mouse brain using 83989-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83989-4-RR (TPRG1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83989-4-RR

    Affinity and Kinetic Characterization of 83989-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83989-4-RR against Human TPRG1L were performed. The affinity constant is 0.234 nM.

TPRG1L Recombinant antibody

TPRG1L Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83989-4-RR

产品说明书

CloneNo. 241070G6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

241070G6, FAM79A, h mover, Mossy fiber terminal-associated vertebrate-specific presynaptic protein, MOVER

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNeuro-2a cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83989-4-RR targets TPRG1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TPRG1L fusion protein Ag22585 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor protein p63 regulated 1-like
别名 241070G6, FAM79A, h mover, Mossy fiber terminal-associated vertebrate-specific presynaptic protein, MOVER
观测分子量 31 kDa
GenBank蛋白编号BC019034
基因名称 TPRG1L
Gene ID (NCBI) 127262
RRIDAB_3671565
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ5T0D9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TPRG1L (Tumor protein p63-regulated gene 1-like protein) belongs to the presynaptic protein involved in the synaptic transmission tuning. It can regulate synaptic release probability by decreasing the calcium sensitivity of release. TPRG1L has two isoforms with molecular weights of 30kd and 23kd respectively. This antibody can recognize the 30kd isoform and may also recognize the 23kd isoform.

实验方案

Product Specific Protocols
WB protocol for TPRG1L antibody 83989-4-RRDownload protocol
IHC protocol for TPRG1L antibody 83989-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols