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CALCOCO1 Recombinant antibody

CALCOCO1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241127G7

Cat no : 84009-4-RR

Print datasheet

Synonyms

PP13275, Coiled-coil coactivator protein, Cocoa, Calphoglin, Calcium-binding and coiled-coil domain-containing protein 1

验证数据展示

  • WB analysis using 84009-4-RR

    WB analysis using 84009-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84009-4-RR (CALCOCO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat testis using 84009-4-RR

    IHC staining of rat testis using 84009-4-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 84009-4-RR

    IHC staining of mouse testis using 84009-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84009-4-RR

    Affinity and Kinetic Characterization of 84009-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84009-4-RR against Human CALCOCO1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHT-1080 cells, MCF-7 cells, HeLa cells, HEK-293T cells, Ramos cells, rat brain tissue
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84009-4-RR is CALCOCO1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CALCOCO1 fusion protein Ag13876 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name calcium binding and coiled-coil domain 1
Synonyms PP13275, Coiled-coil coactivator protein, Cocoa, Calphoglin, Calcium-binding and coiled-coil domain-containing protein 1
Calculated Molecular Weight 691 aa, 77 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC003177
Gene Symbol CALCOCO1
Gene ID (NCBI) 57658
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9P1Z2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CALCOCO1 antibody 84009-4-RRDownload protocol
IHC protocol for CALCOCO1 antibody 84009-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols