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验证数据展示

  • WB analysis using 84009-4-RR

    WB analysis using 84009-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84009-4-RR (CALCOCO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat testis using 84009-4-RR

    IHC staining of rat testis using 84009-4-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 84009-4-RR

    IHC staining of mouse testis using 84009-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84009-4-RR

    Affinity and Kinetic Characterization of 84009-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84009-4-RR against Human CALCOCO1 were performed. The affinity constant is below 1 pM.

CALCOCO1 Recombinant antibody

CALCOCO1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84009-4-RR

产品说明书

CloneNo. 241127G7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

PP13275, Coiled-coil coactivator protein, Cocoa, Calphoglin, Calcium-binding and coiled-coil domain-containing protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-1080 cells, MCF-7 cells, HeLa cells, HEK-293T cells, Ramos cells, rat brain tissue
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84009-4-RR targets CALCOCO1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CALCOCO1 fusion protein Ag13876 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calcium binding and coiled-coil domain 1
别名 PP13275, Coiled-coil coactivator protein, Cocoa, Calphoglin, Calcium-binding and coiled-coil domain-containing protein 1
计算分子量 691 aa, 77 kDa
观测分子量100 kDa
GenBank蛋白编号BC003177
基因名称 CALCOCO1
Gene ID (NCBI) 57658
RRIDAB_3671577
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9P1Z2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CALCOCO1 antibody 84009-4-RRDownload protocol
IHC protocol for CALCOCO1 antibody 84009-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols