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KIF5A Recombinant antibody, PBS Only (Capture)

KIF5A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241124H1

Cat no : 84013-4-PBS

Print datasheet

Synonyms

Neuronal kinesin heavy chain, MY050, Kinesin heavy chain neuron-specific 1, Kinesin heavy chain isoform 5A, D12S1889

验证数据展示

  • WB analysis of rat brain using 84013-4-RR (same clone as 84013-4-PBS)

    WB analysis of rat brain using 84013-4-RR (same clone as 84013-4-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 84013-4-RR (KIF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

  • IF Staining of PC-12 using 84013-4-RR (same clone as 84013-4-PBS)

    IF Staining of PC-12 using 84013-4-RR (same clone as 84013-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using KIF5A antibody (84013-4-RR, Clone: 241124H1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 84013-4-RR (same clone as 84013-4-PBS)

    FC experiment of K-562 using 84013-4-RR (same clone as 84013-4-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-4-RR, Clone:241124H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 84013-4-RR (same clone as 84013-4-PBS)

    FC experiment of SH-SY5Y using 84013-4-RR (same clone as 84013-4-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-4-RR, Clone:241124H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84013-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00967-1

    Cytometric bead array standard curve of MP00967-1

    Cytometric bead array standard curve of MP00967-1, KIF5A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84013-4-PBS. Detection antibody: 84013-3-PBS. Standard: Ag15518. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00967-2

    Cytometric bead array standard curve of MP00967-2

    Cytometric bead array standard curve of MP00967-2, KIF5A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84013-4-PBS. Detection antibody: 84013-1-PBS. Standard: Ag15518. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84013-4-RR

    Affinity and Kinetic Characterization of 84013-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84013-4-RR against Human KIF5A were performed. The affinity constant is 78.9 pM.

产品信息

84013-4-PBS targets KIF5A as part of a matched antibody pair:

MP00967-1: 84013-4-PBS capture and 84013-3-PBS detection (validated in Cytometric bead array)

MP00967-2: 84013-4-PBS capture and 84013-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen KIF5A fusion protein Ag15518 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name kinesin family member 5A
Synonyms Neuronal kinesin heavy chain, MY050, Kinesin heavy chain neuron-specific 1, Kinesin heavy chain isoform 5A, D12S1889
Calculated Molecular Weight 1032 aa, 117 kDa
Observed Molecular Weight 118-120 kDa
GenBank Accession NumberBC146670
Gene Symbol KIF5A
Gene ID (NCBI) 3798
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12840
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.