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验证数据展示

  • WB analysis of SH-SY5Y using 84013-5-RR

    WB analysis of SH-SY5Y using 84013-5-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84013-5-RR (KIF5A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using 84013-5-RR

    FC experiment of SH-SY5Y using 84013-5-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-5-RR, Clone:241124H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of K562 using 84013-5-RR

    FC experiment of K562 using 84013-5-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug KIF5A Recombinant antibody (84013-5-RR, Clone:241124H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84013-5-RR

    Affinity and Kinetic Characterization of 84013-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84013-5-RR against Human KIF5A were performed. The affinity constant is 0.773 nM.

KIF5A Recombinant antibody

KIF5A Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84013-5-RR

产品说明书

CloneNo. 241124H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Neuronal kinesin heavy chain, MY050, Kinesin heavy chain neuron-specific 1, Kinesin heavy chain isoform 5A, D12S1889

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, U-87-MG cells, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inK562, SH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84013-5-RR targets KIF5A in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 KIF5A fusion protein Ag15518 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kinesin family member 5A
别名 Neuronal kinesin heavy chain, MY050, Kinesin heavy chain neuron-specific 1, Kinesin heavy chain isoform 5A, D12S1889
计算分子量 1032 aa, 117 kDa
观测分子量 118-120 kDa
GenBank蛋白编号BC146670
基因名称 KIF5A
Gene ID (NCBI) 3798
RRIDAB_3671582
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ12840
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.

实验方案

Product Specific Protocols
WB protocol for KIF5A antibody 84013-5-RRDownload protocol
FC protocol for KIF5A antibody 84013-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols