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验证数据展示

  • WB analysis using 84023-1-RR

    WB analysis using 84023-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84023-1-RR (CSF2RA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 84023-1-RR

    IHC staining of human placenta using 84023-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84023-1-RR (CSF2RA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 84023-1-RR

    IHC staining of human placenta using 84023-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84023-1-RR (CSF2RA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of THP-1 using 84023-1-RR

    IF Staining of THP-1 using 84023-1-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CSF2RA antibody (84023-1-RR, Clone: 241266A9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84023-1-RR

    Affinity and Kinetic Characterization of 84023-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84023-1-RR against Human CSF2RA/CD116 were performed. The affinity constant is 1.05 nM.

CSF2RA/CD116 Recombinant antibody

CSF2RA/CD116 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84023-1-RR

产品说明书

CloneNo. 241266A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CD116, 241266A9, GMCSFR, GM CSF R alpha, CSF2RA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, HL-60 cells, U-937 cells, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84023-1-RR targets CSF2RA/CD116 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
别名 CD116, 241266A9, GMCSFR, GM CSF R alpha, CSF2RA
计算分子量 46 kDa
观测分子量60-70 kDa
GenBank蛋白编号BC002635
基因名称 CD116
Gene ID (NCBI) 1438
RRIDAB_3671592
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP15509
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD116, also named as CSF2RA CDw116 CSF2RAX CSF2RAY CSF2RX CSF2RY GM-CSF-R-alpha GMCSFR and GMR, is a low affinity receptor for granulocyte-macrophage colony-stimulating factor. CD116 transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.

实验方案

Product Specific Protocols
WB protocol for CSF2RA/CD116 antibody 84023-1-RRDownload protocol
IHC protocol for CSF2RA/CD116 antibody 84023-1-RRDownload protocol
IF protocol for CSF2RA/CD116 antibody 84023-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols