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HINT2 Recombinant antibody

HINT2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241150D10

Cat no : 84031-5-RR

Print datasheet

Synonyms

HINT-2, HINT 3, HINT 2, EC:3.9.1.-, Adenosine 5'-monophosphoramidase HINT2

验证数据展示

  • WB analysis using 84031-5-RR

    WB analysis using 84031-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84031-5-RR (HINT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84031-5-RR

    IF Staining of HeLa using 84031-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HINT2 antibody (84031-5-RR, Clone: 241150D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), (red).

  • Affinity and Kinetic Characterization of 84031-5-RR

    Affinity and Kinetic Characterization of 84031-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84031-5-RR against Human HINT2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, HuH-7 cells, mouse liver tissue, rat liver tissue, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84031-5-RR targets HINT2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HINT2 fusion protein Ag12413 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histidine triad nucleotide binding protein 2
Synonyms HINT-2, HINT 3, HINT 2, EC:3.9.1.-, Adenosine 5'-monophosphoramidase HINT2
Calculated Molecular Weight 163 aa, 17 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC047737
Gene Symbol HINT2
Gene ID (NCBI) 84681
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BX68
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HINT2 antibody 84031-5-RRDownload protocol
IF protocol for HINT2 antibody 84031-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols