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验证数据展示

  • IHC staining of mouse colon using 84033-2-RR (same clone as 84033-2-PBS)

    IHC staining of mouse colon using 84033-2-RR (same clone as 84033-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84033-2-RR (ACSF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84033-2-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 84033-2-RR (same clone as 84033-2-PBS)

    IF Staining of A549 using 84033-2-RR (same clone as 84033-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ACSF2 antibody (84033-2-RR, Clone: 241208C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84033-2-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 84033-2-RR (same clone as 84033-2-PBS)

    IF Staining of A549 using 84033-2-RR (same clone as 84033-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ACSF2 antibody (84033-2-RR, Clone: 241208C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84033-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00952-1

    Cytometric bead array standard curve of MP00952-1

    Cytometric bead array standard curve of MP00952-1, ACSF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84033-2-PBS. Detection antibody: 84033-3-PBS. Standard: Ag9199. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00952-2

    Cytometric bead array standard curve of MP00952-2

    Cytometric bead array standard curve of MP00952-2, ACSF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84033-1-PBS. Detection antibody: 84033-2-PBS. Standard: Ag9199. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84033-2-PBS

    Affinity and Kinetic Characterization of 84033-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 84033-2-PBS against Human ACSF2 was performed. The affinity constant is 0.221 nM.

  • Affinity and Kinetic Characterization of 84033-2-RR

    Affinity and Kinetic Characterization of 84033-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84033-2-RR against Human ACSF2 were performed. The affinity constant is 0.595 nM.

ACSF2 Recombinant antibody, PBS Only (Capture/Detector)

ACSF2 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84033-2-PBS

产品说明书

CloneNo. 241208C3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

UNQ493/PRO1009, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, EC:6.2.1.2, ACSF 2, 241208C3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84033-2-PBS targets ACSF2 as part of a matched antibody pair:

MP00952-1: 84033-2-PBS capture and 84033-3-PBS detection (validated in Cytometric bead array)

MP00952-2: 84033-1-PBS capture and 84033-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 ACSF2 fusion protein Ag9199 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acyl-CoA synthetase family member 2
别名 UNQ493/PRO1009, Medium-chain acyl-CoA ligase ACSF2, mitochondrial, EC:6.2.1.2, ACSF 2, 241208C3
计算分子量 615 aa, 68 kDa
GenBank蛋白编号BC012053
基因名称 ACSF2
Gene ID (NCBI) 80221
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96CM8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C