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验证数据展示

  • WB analysis using 84041-2-RR (same clone as 84041-2-PBS)

    WB analysis using 84041-2-RR (same clone as 84041-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84041-2-RR (PRC1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

    IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

    IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

    IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

    IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

    IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

    IF Staining of HepG2 using 84041-2-RR (same clone as 84041-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 84041-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00970-1

    Cytometric bead array standard curve of MP00970-1

    Cytometric bead array standard curve of MP00970-1, PRC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84041-2-PBS. Detection antibody: 84041-1-PBS. Standard: Ag8028. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00970-2

    Cytometric bead array standard curve of MP00970-2

    Cytometric bead array standard curve of MP00970-2, PRC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84041-2-PBS. Detection antibody: 84041-3-PBS. Standard:null. Range: null.

  • Cytometric bead array standard curve of MP00970-2

    Cytometric bead array standard curve of MP00970-2

    Cytometric bead array standard curve of MP00970-2, PRC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84041-2-PBS. Detection antibody: 84041-3-PBS. Standard: Ag8028. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84041-2-RR

    Affinity and Kinetic Characterization of 84041-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84041-2-RR against Human PRC1 were performed. The affinity constant is below 1 pM.

PRC1 Recombinant antibody, PBS Only (Capture)

PRC1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84041-2-PBS

产品说明书

CloneNo. 241216H2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

ASE1, 241216H2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84041-2-PBS targets PRC1 as part of a matched antibody pair:

MP00970-1: 84041-2-PBS capture and 84041-1-PBS detection (validated in Cytometric bead array)

MP00970-2: 84041-2-PBS capture and 84041-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 PRC1 fusion protein Ag8028 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein regulator of cytokinesis 1
别名 ASE1, 241216H2
计算分子量 72 kDa
GenBank蛋白编号BC003138
基因名称 PRC1
Gene ID (NCBI) 9055
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43663
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively.