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验证数据展示

  • WB analysis using 84054-5-RR (same clone as 84054-5-PBS)

    WB analysis using 84054-5-RR (same clone as 84054-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84054-5-RR (GPAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84054-5-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 84054-5-RR (same clone as 84054-5-PBS)

    WB analysis of HepG2 using 84054-5-RR (same clone as 84054-5-PBS)

    WB result of GPAM antibody (84054-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPAM transfected HepG2 cells. This data was developed using the same antibody clone with 84054-5-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00994-2

    Cytometric bead array standard curve of MP00994-2

    Cytometric bead array standard curve of MP00994-2, GPAM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84054-5-PBS. Detection antibody: 84054-1-PBS. Standard: Ag3120. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84054-5-PBS

    Affinity and Kinetic Characterization of 84054-5-PBS

    Biolayer interferometry (BLl) kinetic assay of 84054-5-PBS against Human GPAM was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 84054-5-RR

    Affinity and Kinetic Characterization of 84054-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84054-5-RR against Human GPAM were performed. The affinity constant is below 1 pM.

GPAM Recombinant antibody, PBS Only (Capture)

GPAM Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 84054-5-PBS

产品说明书

CloneNo. 241144H6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Cytometric bead array, Indirect ELISA

别名

241144H6, EC:2.3.1.15, Glycerol-3-phosphate acyltransferase 1, mitochondrial, GPAT 1, GPAT1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84054-5-PBS targets GPAM as part of a matched antibody pair:

MP00994-2: 84054-5-PBS capture and 84054-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GPAM fusion protein Ag3120 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glycerol-3-phosphate acyltransferase, mitochondrial
别名 241144H6, EC:2.3.1.15, Glycerol-3-phosphate acyltransferase 1, mitochondrial, GPAT 1, GPAT1
计算分子量 828 aa, 94 kDa
观测分子量 90-92 kDa
GenBank蛋白编号BC030783
基因名称 GPAM
Gene ID (NCBI) 57678
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9HCL2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPAM (Glycerol-3-Phosphate Acyltransferase, Mitochondrial) is a Protein Coding gene. This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene.Diseases associated with GPAM include Rhizomelic Chondrodysplasia Punctata, Type 2 and Congenital Generalized Lipodystrophy. GPAM can be detected as 90-92 kDa.