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GPAM Recombinant antibody, PBS Only (Capture)

GPAM Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241144H6

Cat no : 84054-5-PBS

Print datasheet

Synonyms

GPAT-1, GPAT1, GPAT 1, Glycerol-3-phosphate acyltransferase 1, mitochondrial, EC:2.3.1.15

验证数据展示

  • WB analysis using 84054-5-RR (same clone as 84054-5-PBS)

    WB analysis using 84054-5-RR (same clone as 84054-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84054-5-RR (GPAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84054-5-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00994-2

    Cytometric bead array standard curve of MP00994-2

    Cytometric bead array standard curve of MP00994-2, GPAM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84054-5-PBS. Detection antibody: 84054-1-PBS. Standard: Ag3120. Range: 0.625-80 ng/mL.

产品信息

84054-5-PBS targets GPAM as part of a matched antibody pair:

MP00994-2: 84054-5-PBS capture and 84054-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GPAM fusion protein Ag3120 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glycerol-3-phosphate acyltransferase, mitochondrial
Synonyms GPAT-1, GPAT1, GPAT 1, Glycerol-3-phosphate acyltransferase 1, mitochondrial, EC:2.3.1.15
Calculated Molecular Weight 828 aa, 94 kDa
Observed Molecular Weight 90-92 kDa
GenBank Accession NumberBC030783
Gene Symbol GPAM
Gene ID (NCBI) 57678
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HCL2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPAM (Glycerol-3-Phosphate Acyltransferase, Mitochondrial) is a Protein Coding gene. This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene.Diseases associated with GPAM include Rhizomelic Chondrodysplasia Punctata, Type 2 and Congenital Generalized Lipodystrophy. GPAM can be detected as 90-92 kDa.