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MIRO1 Recombinant antibody

MIRO1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241157D1

Cat no : 84055-5-RR

Print datasheet

Synonyms

RHOT1, Rac-GTP-binding protein-like protein, MIRO 1, hMiro 1, EC:3.6.5.-

验证数据展示

  • WB analysis using 84055-5-RR

    WB analysis using 84055-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84055-5-RR (MIRO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 84055-5-RR

    IHC staining of mouse kidney using 84055-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84055-5-RR (MIRO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84055-5-RR

    Affinity and Kinetic Characterization of 84055-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83945-5-RR against Human RHOT1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HL-60 cells, mouse testis tissue, mouse heart tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84055-5-RR is MIRO1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MIRO1 fusion protein Ag15605 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ras homolog gene family, member T1
Synonyms RHOT1, Rac-GTP-binding protein-like protein, MIRO 1, hMiro 1, EC:3.6.5.-
Calculated Molecular Weight 618 aa, 71 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC060781
Gene Symbol MIRO1
Gene ID (NCBI) 55288
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IXI2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MIRO1 antibody 84055-5-RRDownload protocol
IHC protocol for MIRO1 antibody 84055-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols