• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84090-4-RR

    WB analysis using 84090-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84090-4-RR (MYO9B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 84090-4-RR

    FC experiment of A431 using 84090-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MYO9B Recombinant antibody (84090-4-RR, Clone:241163D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

MYO9B Recombinant antibody

MYO9B Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84090-4-RR

产品说明书

CloneNo. 241163D8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Unconventional myosin-9b, MYR5, myosin IXB, MYO9B variant protein, CELIAC4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells, HL-60 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy MYO9B Ready-To-Use IHC Kit. MYO9B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84090-4-RR targets MYO9B in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 MYO9B fusion protein Ag33349 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myosin IXB
别名 Unconventional myosin-9b, MYR5, myosin IXB, MYO9B variant protein, CELIAC4
计算分子量 2023 aa, 223 kDa
观测分子量 230-250 kDa
GenBank蛋白编号BC018108
基因名称 MYO9B
Gene ID (NCBI) 4650
RRIDAB_3671656
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13459
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYO9B antibody 84090-4-RRDownload protocol
FC protocol for MYO9B antibody 84090-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols