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MYO9B Recombinant antibody

MYO9B Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241163D8

Cat no : 84090-4-RR

Print datasheet

Synonyms

Unconventional myosin-9b, MYR5, myosin IXB, MYO9B variant protein, CELIAC4

验证数据展示

  • WB analysis using 84090-4-RR

    WB analysis using 84090-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84090-4-RR (MYO9B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 84090-4-RR

    FC experiment of A431 using 84090-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MYO9B Recombinant antibody (84090-4-RR, Clone:241163D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells, HL-60 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy MYO9B Ready-To-Use IHC Kit. MYO9B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84090-4-RR is MYO9B Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen MYO9B fusion protein Ag33349 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myosin IXB
Synonyms Unconventional myosin-9b, MYR5, myosin IXB, MYO9B variant protein, CELIAC4
Calculated Molecular Weight 2023 aa, 223 kDa
Observed Molecular Weight 230-250 kDa
GenBank Accession NumberBC018108
Gene Symbol MYO9B
Gene ID (NCBI) 4650
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13459
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYO9B antibody 84090-4-RRDownload protocol
FC protocol for MYO9B antibody 84090-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols