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验证数据展示

  • WB analysis using 84104-2-RR

    WB analysis using 84104-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84104-2-RR (RBM10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 84104-2-RR

    WB analysis of rat brain using 84104-2-RR

    rat brain tissue were subjected to SDS PAGE followed by western blot with 84104-2-RR (RBM10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84104-2-RR

    IHC staining of mouse brain using 84104-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-2-RR (RBM10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RBM10 antibody included
  • IHC staining of mouse brain using 84104-2-RR

    IHC staining of mouse brain using 84104-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-2-RR (RBM10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RBM10 antibody included
  • IHC staining of mouse brain using 84104-2-RR

    IHC staining of mouse brain using 84104-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84104-2-RR (RBM10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RBM10 antibody included
  • IF Staining of A431 using 84104-2-RR

    IF Staining of A431 using 84104-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM10 antibody (84104-2-RR, Clone: 241151D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A431 using 84104-2-RR

    FC experiment of A431 using 84104-2-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug RBM10 Recombinant antibody (84104-2-RR, Clone:241151D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84104-2-RR

    Affinity and Kinetic Characterization of 84104-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84104-2-RR against Human RBM10 were performed. The affinity constant is 3.93 nM.

RBM10 Recombinant antibody

RBM10 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84104-2-RR

产品说明书

CloneNo. 241151D7

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9, DXS8237E

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, MCF-7 cells, MDA-MB-231 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy RBM10 Ready-To-Use IHC Kit. RBM10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84104-2-RR targets RBM10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原 RBM10 fusion protein Ag5788 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RNA binding motif protein 10
别名 KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9, DXS8237E
计算分子量 104 kDa
观测分子量100-120 kDa
GenBank蛋白编号BC024153
基因名称 RBM10
Gene ID (NCBI) 8241
RRIDAB_3671670
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP98175
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .

实验方案

Product Specific Protocols
WB protocol for RBM10 antibody 84104-2-RRDownload protocol
IHC protocol for RBM10 antibody 84104-2-RRDownload protocol
IF protocol for RBM10 antibody 84104-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols