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验证数据展示

  • WB analysis using 84107-2-RR

    WB analysis using 84107-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84107-2-RR (PROS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of DU 145 using 84107-2-RR

    WB analysis of DU 145 using 84107-2-RR

    WB result of PROS1 antibody (84107-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PROS1 transfected DU 145 cells.

  • FC experiment of A549 using 84107-2-RR

    FC experiment of A549 using 84107-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PROS1 Recombinant antibody (84107-2-RR, Clone:241241E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of mouse brain using 84107-2-RR

    IHC staining of mouse brain using 84107-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84107-2-RR (PROS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 84107-2-RR

    IHC staining of mouse brain using 84107-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84107-2-RR (PROS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 84107-2-RR

    IF Staining of HepG2 using 84107-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PROS1 antibody (84107-2-RR, Clone: 241241E5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HepG2 using 84107-2-RR

    IF Staining of HepG2 using 84107-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PROS1 antibody (84107-2-RR, Clone: 241241E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84107-2-RR

    Affinity and Kinetic Characterization of 84107-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84107-2-RR against Human PROS1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

PROS1 Recombinant antibody

PROS1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84107-2-RR

产品说明书

CloneNo. 241241E5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

Vitamin K-dependent protein S, PROS, 241241E5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDU 145 cells, PC-3 cells, HuH-7 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84107-2-RR targets PROS1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 PROS1 fusion protein Ag10539 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein S (alpha)
别名 Vitamin K-dependent protein S, PROS, 241241E5
计算分子量 75 kDa
观测分子量 75 kDa
GenBank蛋白编号BC015801
基因名称 PROS1
Gene ID (NCBI) 5627
RRIDAB_3671673
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07225
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.

实验方案

Product Specific Protocols
WB protocol for PROS1 antibody 84107-2-RRDownload protocol
IHC protocol for PROS1 antibody 84107-2-RRDownload protocol
IF protocol for PROS1 antibody 84107-2-RRDownload protocol
FC protocol for PROS1 antibody 84107-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols