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验证数据展示

  • WB analysis using 84119-3-RR

    WB analysis using 84119-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84119-3-RR (TRAPPC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 84119-3-RR

    WB analysis of MCF-7 using 84119-3-RR

    WB result of TRAPPC4 antibody (84119-3-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC4 transfected MCF-7 cells.

  • IP experiment of HL-60 using 84119-3-RR

    IP experiment of HL-60 using 84119-3-RR

    IP result of anti-TRAPPC4 (IP:84119-3-RR, 4ug; Detection:84119-3-RR 1:3000) with HL-60 cells lysate 2240 ug.

  • Affinity and Kinetic Characterization of 84119-3-RR

    Affinity and Kinetic Characterization of 84119-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84119-3-RR against Human TRAPPC4 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

TRAPPC4 Recombinant antibody

TRAPPC4 Uni-rAbTM Recombinant Antibody for WB, IP, ELISA
Cat No. 84119-3-RR

产品说明书

CloneNo. 241158C11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

SBDN, PTD009, HSPC172, CGI-104, CGI 104

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, MCF-7 cells, HL-60 cells, RAW 264.7 cells, mouse testis tissue, rat testis tissue
Positive IP detected inHL-60 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84119-3-RR targets TRAPPC4 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TRAPPC4 fusion protein Ag2737 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 trafficking protein particle complex 4
别名 SBDN, PTD009, HSPC172, CGI-104, CGI 104
计算分子量 219 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC010866
基因名称 TRAPPC4
Gene ID (NCBI) 51399
RRIDAB_3671682
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y296
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC4, also known as synbindin, is a core component of the TRAPP complexes and one of the essential subunits for guanine nucleotide exchange factor activity for Rab1 GTPase (PMID: 31794024). Deficiencies in vesicular transport mediated by TRAPPC4 have been associated with severe syndromic intellectual disability (PMID: 31794024).

实验方案

Product Specific Protocols
WB protocol for TRAPPC4 antibody 84119-3-RRDownload protocol
IP protocol for TRAPPC4 antibody 84119-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols