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NELF-B Recombinant antibody, PBS Only (Capture)

NELF-B Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241434F12

Cat no : 84135-4-PBS

Print datasheet

Synonyms

NELFB, NELF B, Negative elongation factor B, KIAA1182, Cofactor of BRCA1

验证数据展示

  • FC experiment of A431 using 84135-4-RR (same clone as 84135-4-PBS)

    FC experiment of A431 using 84135-4-RR (same clone as 84135-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug NELF-B Recombinant antibody (84135-4-RR, Clone:241434F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84135-4-PBS in a different storage buffer formulation.

  • WB analysis using 84135-4-RR (same clone as 84135-4-PBS)

    WB analysis using 84135-4-RR (same clone as 84135-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84135-4-RR (NELF-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84135-4-PBS in a different storage buffer formulation.

  • FC experiment of BxPC-3 using 84135-4-RR (same clone as 84135-4-PBS)

    FC experiment of BxPC-3 using 84135-4-RR (same clone as 84135-4-PBS)

    1x10^6 BxPC-3 cells were intracellularly stained with 0.25 ug NELF-B Recombinant antibody (84135-4-RR, Clone:241434F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84135-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01055-1

    Cytometric bead array standard curve of MP01055-1

    Cytometric bead array standard curve of MP01055-1, NELF-B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84135-4-PBS. Detection antibody: 84135-1-PBS. Standard: Ag9599. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01055-3

    Cytometric bead array standard curve of MP01055-3

    Cytometric bead array standard curve of MP01055-3, NELF-B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84135-4-PBS. Detection antibody: 84135-3-PBS. Standard: Ag9599. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84135-4-RR

    Affinity and Kinetic Characterization of 84135-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84135-4-RR against Human NELF-B were performed. The affinity constant is 0.199 nM.

Planning an IHC experiment? We recommend our IHCeasy NELFB Ready-To-Use IHC Kit. NELFB primary antibody included.

产品信息

84135-4-PBS targets NELF-B as part of a matched antibody pair:

MP01055-1: 84135-4-PBS capture and 84135-1-PBS detection (validated in Cytometric bead array)

MP01055-3: 84135-4-PBS capture and 84135-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen NELF-B fusion protein Ag9599 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cofactor of BRCA1
Synonyms NELFB, NELF B, Negative elongation factor B, KIAA1182, Cofactor of BRCA1
Calculated Molecular Weight580aa,66 kDa; 475aa,54 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC011892
Gene Symbol NELFB
Gene ID (NCBI) 25920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8WX92
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cofactor of BRCA1 (COBRA1) was first identified as a BRCA1-interacting protein and subsequently found to be the B subunit of the negative elongation factor complex (NELF-B). Cobra1 is essential for early embryogenesis (PMID: 19340312). The MW of this protein is 66 kDa, and this antibody specially recognises the 66 kDa protein.