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验证数据展示

  • WB analysis using 84162-3-RR

    WB analysis using 84162-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84162-3-RR (PDCD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 84162-3-RR

    FC experiment of HeLa using 84162-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PDCD4 Recombinant antibody (84162-3-RR, Clone:241463E3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84162-3-RR

    Affinity and Kinetic Characterization of 84162-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84162-3-RR against Human PDCD4 were performed. The affinity constant is 1.54 nM.

PDCD4 Recombinant antibody

PDCD4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84162-3-RR

产品说明书

CloneNo. 241463E3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Tis, PDCD 4, Nuclear antigen H731-like, H731, Dug

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells, HUVEC cells, MCF-7 cells, HEK-293 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PDCD4 Ready-To-Use IHC Kit. PDCD4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84162-3-RR targets PDCD4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 PDCD4 fusion protein Ag3288 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 programmed cell death 4 (neoplastic transformation inhibitor)
别名 Tis, PDCD 4, Nuclear antigen H731-like, H731, Dug
计算分子量 469 aa, 52 kDa
观测分子量 54-64 kDa
GenBank蛋白编号BC026104
基因名称 PDCD4
Gene ID (NCBI) 27250
RRIDAB_3671724
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ53EL6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A. The calculated molecular wight of unmodified PDCD4 protein is 52 kDa, but the modified protein is about 54-64 kDa.

实验方案

Product Specific Protocols
WB protocol for PDCD4 antibody 84162-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols