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验证数据展示

  • WB analysis using 84201-1-RR

    WB analysis using 84201-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84201-1-RR (MRPS18A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 84201-1-RR

    WB analysis of HepG2 using 84201-1-RR

    WB result of MRPS18A antibody (84201-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS18A transfected HepG2 cells.

  • Affinity and Kinetic Characterization of 84201-1-RR

    Affinity and Kinetic Characterization of 84201-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84201-1-RR against Human MRPS18A were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

MRPS18A Recombinant antibody

MRPS18A Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84201-1-RR

产品说明书

CloneNo. 240850C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

MRP S18 3, Large ribosomal subunit protein bS18a, HumanS18b, 39S ribosomal protein S18a, mitochondrial, 39S ribosomal protein S18-3, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, A549 cells, HeLa cells, L02 cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84201-1-RR targets MRPS18A in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 MRPS18A fusion protein Ag9011 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitochondrial ribosomal protein S18A
别名 MRP S18 3, Large ribosomal subunit protein bS18a, HumanS18b, 39S ribosomal protein S18a, mitochondrial, 39S ribosomal protein S18-3, mitochondrial
计算分子量 196 aa, 22 kDa
观测分子量15 kDa
GenBank蛋白编号BC010364
基因名称 MRPS18A
Gene ID (NCBI) 55168
RRIDAB_3671757
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NVS2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MRPS18A (Mitochondrial Ribosomal Protein S18A) is a protein coding gene. It encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The increased expression of MRPS18A in cancer cells is explained by the increased energy metabolism of cancer cells and may pave the way for new diagnostic and therapeutic avenues to be explored (PMID: 28056857).

实验方案

Product Specific Protocols
WB protocol for MRPS18A antibody 84201-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols