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验证数据展示

  • WB analysis using 84230-2-RR (same clone as 84230-2-PBS)

    WB analysis using 84230-2-RR (same clone as 84230-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84230-2-RR (NPEPPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84230-2-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 84230-2-RR (same clone as 84230-2-PBS)

    IF Staining of A549 using 84230-2-RR (same clone as 84230-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using NPEPPS antibody (84230-2-RR, Clone: 241502G8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84230-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01126-2

    Cytometric bead array standard curve of MP01126-2

    Cytometric bead array standard curve of MP01126-2, NPEPPS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84230-2-PBS. Detection antibody: 84230-3-PBS. Standard: Ag36035. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84230-2-PBS

    Affinity and Kinetic Characterization of 84230-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 84230-2-PBS against Human NPEPPS was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 84230-2-RR

    Affinity and Kinetic Characterization of 84230-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84230-2-RR against Human NPEPPS were performed. The affinity constant is below 1 pM.

NPEPPS Recombinant antibody, PBS Only (Capture)

NPEPPS Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84230-2-PBS

产品说明书

CloneNo. 241502G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

PSA, MP100, AAP S, 241502G8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84230-2-PBS targets NPEPPS as part of a matched antibody pair:

MP01126-2: 84230-2-PBS capture and 84230-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NPEPPS fusion protein Ag36035 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aminopeptidase puromycin sensitive
别名 PSA, MP100, AAP S, 241502G8
观测分子量103 kDa
GenBank蛋白编号BC065294
基因名称 NPEPPS
Gene ID (NCBI) 9520
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55786
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NPEPPS, also known as PSA, is a member of the oxytocinase subfamily of the M1 aminopeptidase family. NPEPPS encodes puromycin-sensitive aminopeptidase, a zinc metallopeptidase that hydrolyzes amino acids from the N-terminus of its substrate. The protein has 2 isoforms and is located in the cytoplasm.