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验证数据展示

  • WB analysis using 84245-2-RR (same clone as 84245-2-PBS)

    WB analysis using 84245-2-RR (same clone as 84245-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84245-2-RR (CD84 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84245-2-PBS in a different storage buffer formulation.

  • IF Staining of Raji using 84245-2-RR (same clone as 84245-2-PBS)

    IF Staining of Raji using 84245-2-RR (same clone as 84245-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD84 antibody (84245-2-RR, Clone: 241342F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84245-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01112-1

    Cytometric bead array standard curve of MP01112-1

    Cytometric bead array standard curve of MP01112-1, CD84 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98108-1-PBS. Detection antibody: 84245-2-PBS. Standard: RP02347. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01112-3

    Cytometric bead array standard curve of MP01112-3

    Cytometric bead array standard curve of MP01112-3, CD84 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84245-3-PBS. Detection antibody: 84245-2-PBS. Standard: RP02347. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84245-2-RR

    Affinity and Kinetic Characterization of 84245-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84245-2-RR against Human CD84 were performed. The affinity constant is 0.409 nM.

CD84 Recombinant antibody, PBS Only (Detector)

CD84 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84245-2-PBS

产品说明书

CloneNo. 241342F3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Hly9-beta, Hly9 beta, hCD84, Cell surface antigen MAX.3, CD84 molecule

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84245-2-PBS targets CD84 as part of a matched antibody pair:

MP01112-1: 98108-1-PBS capture and 84245-2-PBS detection (validated in Cytometric bead array)

MP01112-3: 84245-3-PBS capture and 84245-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD84 molecule
别名 Hly9-beta, Hly9 beta, hCD84, Cell surface antigen MAX.3, CD84 molecule
计算分子量 345 aa, 39 kDa
观测分子量70 kDa
GenBank蛋白编号BC020063
基因名称 CD84
Gene ID (NCBI) 8832
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UIB8
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD84, also known as SLAMF5, is a member of the CD2 subfamily of the immunoglobulin receptor superfamily (PMID: 9310491). CD84 is a single-chain type-I glycoprotein composed of two extracellular Ig-like domains, a hydrophobic transmembrane region, and a cytoplasmic domain. It is broadly expressed on almost all leukocyte subsets. CD84 functions as a homophilic adhesion molecule, whose signaling can activate or inhibit leukocyte function depending on the cell type and its stage of activation or differentiation (PMID: 30522694).