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CD84 Recombinant antibody, PBS Only (Detector)

CD84 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241342F3

Cat no : 84245-2-PBS

Print datasheet

Synonyms

Hly9-beta, Hly9 beta, hCD84, Cell surface antigen MAX.3, CD84 molecule

验证数据展示

  • WB analysis using 84245-2-RR (same clone as 84245-2-PBS)

    WB analysis using 84245-2-RR (same clone as 84245-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84245-2-RR (CD84 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84245-2-PBS in a different storage buffer formulation.

  • IF Staining of Raji using 84245-2-RR (same clone as 84245-2-PBS)

    IF Staining of Raji using 84245-2-RR (same clone as 84245-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD84 antibody (84245-2-RR, Clone: 241342F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84245-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01112-1

    Cytometric bead array standard curve of MP01112-1

    Cytometric bead array standard curve of MP01112-1, CD84 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98108-1-PBS. Detection antibody: 84245-2-PBS. Standard: RP02347. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01112-3

    Cytometric bead array standard curve of MP01112-3

    Cytometric bead array standard curve of MP01112-3, CD84 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84245-3-PBS. Detection antibody: 84245-2-PBS. Standard: RP02347. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84245-2-RR

    Affinity and Kinetic Characterization of 84245-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84245-2-RR against Human CD84 were performed. The affinity constant is 0.409 nM.

产品信息

84245-2-PBS targets CD84 as part of a matched antibody pair:

MP01112-1: 98108-1-PBS capture and 84245-2-PBS detection (validated in Cytometric bead array)

MP01112-3: 84245-3-PBS capture and 84245-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD84 molecule
Synonyms Hly9-beta, Hly9 beta, hCD84, Cell surface antigen MAX.3, CD84 molecule
Calculated Molecular Weight 345 aa, 39 kDa
GenBank Accession NumberBC020063
Gene Symbol CD84
Gene ID (NCBI) 8832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UIB8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD84, also known as SLAMF5, is a member of the CD2 subfamily of the immunoglobulin receptor superfamily (PMID: 9310491). CD84 is a single-chain type-I glycoprotein composed of two extracellular Ig-like domains, a hydrophobic transmembrane region, and a cytoplasmic domain. It is broadly expressed on almost all leukocyte subsets. CD84 functions as a homophilic adhesion molecule, whose signaling can activate or inhibit leukocyte function depending on the cell type and its stage of activation or differentiation (PMID: 30522694).