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VCAM-1/CD106 Recombinant antibody, PBS Only (Capture)

VCAM-1/CD106 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241284B2

Cat no : 84249-4-PBS

Print datasheet

Synonyms

VCAM1, VCAM-1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM, INCAM-100

验证数据展示

  • WB analysis of HUVEC using 84249-4-RR (same clone as 84249-4-PBS)

    WB analysis of HUVEC using 84249-4-RR (same clone as 84249-4-PBS)

    TNF alpha treated HUVEC cells were subjected to SDS PAGE followed by western blot with 84249-4-RR (VCAM-1/CD106 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84249-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01145-3

    Sandwich ELISA standard curve of MP01145-3

    Sandwich ELISA standard curve of MP01145-3, Human VCAM-1/CD106 Recombinant Matched Antibody Pair - PBS only. 84249-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02345. 84249-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • Sample test of MP01145-3

    Sample test of MP01145-3

    Serum of six individual healthy human donors was measured. The human VCAM-1/CD106 concentration of detected samples was determined to be 188.79 ng/mL with a range of 126.68 - 336.06 ng/mL.

  • Cytometric bead array standard curve of MP01145-3

    Cytometric bead array standard curve of MP01145-3

    Cytometric bead array standard curve of MP01145-3, VCAM-1/CD106 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84249-4-PBS. Detection antibody: 84249-2-PBS. Standard: RP02345. Range: 0.625-40 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy VCAM-1 Ready-To-Use IHC Kit. VCAM-1 primary antibody included.

产品信息

84249-4-PBS targets VCAM-1/CD106 as part of a matched antibody pair:

MP01145-3: 84249-4-PBS capture and 84249-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name vascular cell adhesion molecule 1
Synonyms VCAM1, VCAM-1, Soluble Vascular Cell Adhesion Molecule-1, L1CAM, INCAM-100
Calculated Molecular Weight81 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberNM_001078.4
Gene Symbol VCAM-1
Gene ID (NCBI) 7412
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19320-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).