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Siglec-7/CD328 Recombinant antibody

Siglec-7/CD328 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241525G3

Cat no : 84253-4-RR

Print datasheet

Synonyms

SIGLEC7, Siglec-7, D siglec, CD328, AIRM-1

验证数据展示

  • WB analysis of U-937 using 84253-4-RR

    WB analysis of U-937 using 84253-4-RR

    U-937 cells were subjected to SDS PAGE followed by western blot with 84253-4-RR (Siglec-7/CD328 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84253-4-RR

    IHC staining of human tonsillitis using 84253-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84253-4-RR (Siglec-7/CD328 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84253-4-RR

    IHC staining of human tonsillitis using 84253-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84253-4-RR (Siglec-7/CD328 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84253-4-RR

    Affinity and Kinetic Characterization of 84253-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84253-4-RR against Human Siglec-7/CD328 were performed. The affinity constant is 53.4 nM.

经过测试的应用

Positive WB detected inU-937 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84253-4-RR targets Siglec-7/CD328 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sialic acid binding Ig-like lectin 7
Synonyms SIGLEC7, Siglec-7, D siglec, CD328, AIRM-1
Calculated Molecular Weight51 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberNM_014385.4
Gene Symbol Siglec-7
Gene ID (NCBI) 27036
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y286-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sialic acid binding Ig-like lectin 7 (Siglec-7), also known as CD328 or p75/AIRM-1, is a member of the Siglec family of glycan-recognition proteins. Siglec-7 is a type-I transmembrane protein consisting of three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs (PMID: 32322597; 10567377). It is mainly expressed on immune cells, with low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8+ T cells (PMID: 10567377). Siglec-7 is an inhibitory receptor that negatively regulates the function of NK cells and modulates the immune response through the interaction of sialic acid-containing ligands (PMID: 27312286).

实验方案

Product Specific Protocols
WB protocol for Siglec-7/CD328 antibody 84253-4-RRDownload protocol
IHC protocol for Siglec-7/CD328 antibody 84253-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols