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验证数据展示

  • WB analysis using 84258-7-RR

    WB analysis using 84258-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84258-7-RR (CD69 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84258-7-RR

    Affinity and Kinetic Characterization of 84258-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84258-7-RR against Human CD69 were performed. The affinity constant is 0.776 nM.

CD69 Recombinant antibody

CD69 Recombinant Antibody for WB, ELISA
Cat No. 84258-7-RR

产品说明书

CloneNo. 241552A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

CD69 molecule, BL-AC/P26, BL AC/P26, AIM, Activation inducer molecule

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84258-7-RR targets CD69 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 FusionProtein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD69 molecule
别名 CD69 molecule, BL-AC/P26, BL AC/P26, AIM, Activation inducer molecule
计算分子量23 kDa
观测分子量35 kDa
GenBank蛋白编号NM_001781.2
基因名称 CD69
Gene ID (NCBI) 969
RRIDAB_3671805
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ07108
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).

实验方案

Product Specific Protocols
WB protocol for CD69 antibody 84258-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols