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IVNS1ABP Recombinant antibody

IVNS1ABP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241587B1

Cat no : 84268-1-RR

Print datasheet

Synonyms

Influenza virus NS1A-binding protein, HSPC068, FLARA3, Aryl hydrocarbon receptor-associated protein 3, ARA3

验证数据展示

  • WB analysis using 84268-1-RR

    WB analysis using 84268-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84268-1-RR (IVNS1ABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse ovary using 84268-1-RR

    WB analysis of mouse ovary using 84268-1-RR

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 84268-1-RR (IVNS1ABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84268-1-RR

    IF Staining of HeLa using 84268-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IVNS1ABP antibody (84268-1-RR, Clone: 241587B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 84268-1-RR

    FC experiment of HeLa using 84268-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug IVNS1ABP Recombinant antibody (84268-1-RR, Clone:241587B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84268-1-RR

    Affinity and Kinetic Characterization of 84268-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84268-1-RR against Human IVNS1ABP were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inU-251 cells, mouse ovary tissue, HEK-293 cells, HepG2 cells, HeLa cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84268-1-RR is IVNS1ABP Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen IVNS1ABP fusion protein Ag6412 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name influenza virus NS1A binding protein
Synonyms Influenza virus NS1A-binding protein, HSPC068, FLARA3, Aryl hydrocarbon receptor-associated protein 3, ARA3
Calculated Molecular Weight 72 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC067739
Gene Symbol IVNS1ABP
Gene ID (NCBI) 10625
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6Y0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IVNS1ABP antibody 84268-1-RRDownload protocol
IF protocol for IVNS1ABP antibody 84268-1-RRDownload protocol
FC protocol for IVNS1ABP antibody 84268-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols