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验证数据展示

  • WB analysis using 84268-1-RR

    WB analysis using 84268-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84268-1-RR (IVNS1ABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse ovary using 84268-1-RR

    WB analysis of mouse ovary using 84268-1-RR

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 84268-1-RR (IVNS1ABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 84268-1-RR

    IHC staining of mouse kidney using 84268-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84268-1-RR (IVNS1ABP antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 84268-1-RR

    IHC staining of mouse kidney using 84268-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84268-1-RR (IVNS1ABP antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84268-1-RR

    IF Staining of HeLa using 84268-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IVNS1ABP antibody (84268-1-RR, Clone: 241587B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 84268-1-RR

    FC experiment of HeLa using 84268-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug IVNS1ABP Recombinant antibody (84268-1-RR, Clone:241587B1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84268-1-RR

    Affinity and Kinetic Characterization of 84268-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84268-1-RR against Human IVNS1ABP were performed. The affinity constant is below 1 pM.

IVNS1ABP Recombinant antibody

IVNS1ABP Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84268-1-RR

产品说明书

CloneNo. 241587B1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

Influenza virus NS1A-binding protein, HSPC068, FLARA3, Aryl hydrocarbon receptor-associated protein 3, ARA3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU-251 cells, mouse ovary tissue, HEK-293 cells, HepG2 cells, HeLa cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:125-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84268-1-RR targets IVNS1ABP in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 IVNS1ABP fusion protein Ag6412 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 influenza virus NS1A binding protein
别名 Influenza virus NS1A-binding protein, HSPC068, FLARA3, Aryl hydrocarbon receptor-associated protein 3, ARA3
计算分子量 72 kDa
观测分子量70 kDa
GenBank蛋白编号BC067739
基因名称 IVNS1ABP
Gene ID (NCBI) 10625
RRIDAB_3671815
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y6Y0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IVNS1ABP is involved in many cell functions, including pre-mRNA splicing, the aryl hydrocarbon receptor (AHR) pathway, F-actin organization and protein ubiquitination. It may play a role in mRNA nuclear export.

实验方案

Product Specific Protocols
WB protocol for IVNS1ABP antibody 84268-1-RRDownload protocol
IHC protocol for IVNS1ABP antibody 84268-1-RRDownload protocol
IF protocol for IVNS1ABP antibody 84268-1-RRDownload protocol
FC protocol for IVNS1ABP antibody 84268-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols