验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HT-29 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84275-1-RR targets NSUN4 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | NSUN4 fusion protein Ag31828 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | NOL1/NOP2/Sun domain family, member 4 |
| 别名 | RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4, 241115B7 |
| 计算分子量 | 384 aa, 43 kDa |
| 观测分子量 | ~43 kDa |
| GenBank蛋白编号 | BC014441 |
| 基因名称 | NSUN4 |
| Gene ID (NCBI) | 387338 |
| RRID | AB_3671820 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q96CB9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NSUN4 antibody 84275-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |