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验证数据展示

  • IHC staining of mouse bladder using 84312-5-RR

    IHC staining of mouse bladder using 84312-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 84312-5-RR (MCTS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse bladder using 84312-5-RR

    IHC staining of mouse bladder using 84312-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 84312-5-RR (MCTS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Jurkat using 84312-5-RR

    IF Staining of Jurkat using 84312-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MCTS1 antibody (84312-5-RR, Clone: 241576H1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MCTS1 Recombinant antibody

MCTS1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84312-5-RR

产品说明书

CloneNo. 241576H1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

Multiple copies T-cell malignancies, MCT-1, MCT1, Malignant T-cell-amplified sequence 1, 241576H1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84312-5-RR targets MCTS1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 MCTS1 fusion protein Ag6968 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 malignant T cell amplified sequence 1
别名 Multiple copies T-cell malignancies, MCT-1, MCT1, Malignant T-cell-amplified sequence 1, 241576H1
计算分子量 21 kDa
GenBank蛋白编号BC001013
基因名称 MCTS1
Gene ID (NCBI) 28985
RRIDAB_3671853
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9ULC4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MCTS1 is an anti-oncogene that plays a role in cell cycle regulation. It can reduce cell doubling time and anchor-dependent growth. The G1 transition time and the duration of the G1 / S transition are shortened. It can also promote the release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits (PMID: 10440924).

实验方案

Product Specific Protocols
IHC protocol for MCTS1 antibody 84312-5-RRDownload protocol
IF protocol for MCTS1 antibody 84312-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols